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Description
| Application | The HIS-Select iLAP (integrated Lysis and Affinity Purification) Plate combines the cell lysis and purification steps into one. These plates are coated with a proprietary high-density nickel chelate matrix. This matrix provides high-capacity affinity binding of recombinant fusion proteins with histidine-containing tags. An additional coating contains all of the reagents and chemicals necessary for the lysis of bacterial cells for the purification of recombinant proteins. In addition, it includes protease inhibitors to help prevent the proteolytic breakdown of proteins. These two coatings allow bacterial culture to be added directly to the plate and simultaneously lysis cells and capture target protein. The iLAP plates may be used to confirm expression of the his-tagged target protein, protein-protein interaction assays, and to rapidly screen multiple constructs. The resulting purified protein is compatible with protein assays, SDS-PAGE, Western blotting, and MALDI. |
| Other Notes | Automated methods have been developed and validated for use with iLAP Plates and are available for downloading at www.sigma-aldrich.com/automation. |
| Legal Information | Covered by US Patent No 6,623,655 with additional patents pending by Sigma, and are sold for research use only. Commercial use requires additional licenses. |
| | HIS-Select is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| | iLAP is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| description | polystyrene, flat bottom plate |
| capacity | ≥2 μg/well binding capacity |
| storage temp. | 2-8°C |
Safety
